PReS13-SPK-1277: Role of ß2GPI in APS

b2-Glycoprotein I (b2-GPI), also known as apolipoprotein H, is a 50 kDa protein that was described for the first time in 1961, and in 1968 the first apparently healthy person was identified deficient in this protein. As no function could be attributed to b2-GPI, the protein did not receive much attention and on average a single publication per year appeared in the literature. From 1990 onwards, the interest in this apparently obsolete protein has increased significantly when b2-GPI was identified as the most important antigen in an autoimmune disease called the antiphospholipid syndrome (APS). APS is an auto-immune disease characterized by thrombotic complications in both arteries and veins as well as pregnancyrelated complications in combination with the presence of so-called antiphospholipid antibodies in the plasma of these patients. It is now generally accepted that these auto-antibodies are not directed against negatively charged phospholipids but towards proteins bound to these phospholipids. Animal studies have shown that the most prominent antigen in APS is b2-GPI, a protein with relatively low affinity towards anionic phospholipids. The importance of antibodies against b2-GPI was demonstrated by injection of these antibodies in mice, which resulted in increased thrombus formation when challenged and they showed an increased resorption of fetuses when pregnant. Despite the obvious importance of b2-GPI in the pathophysiology of APS, these in vivo experiments did not reveal a physiological function for this protein. It was already know for a long time that no circulating immune complexes could be detected in plasma of patients with APS, suggesting that the epitope recognized by the auto-antibodies was cryptic. Moreover, different studies have shown that the presence of autoantibodies against domain I of b2-GPI correlates much better with clinical manifestations than auto-antibodies against the whole protein. This assumption was confirmed by the observation that b2-GPI can exist in at least two conformations, a circular conformation and a stretched conformation. In plasma, b2-GPI predominantly circulates in the circular conformation in which N-terminal domain I interacts with the C-terminal domain V. When b2-GPI binds to anionic phospholipids, it is converted into the stretched conformation. This conformation exposes an otherwise cryptic epitope in domain I and the protein can be recognized by the circulating auto-antibodies. In recent years, novel and exciting data have become available that suggest an important function of this protein in innate immunity. b2-GPI was found to scavenge lipopolysaccharide (LPS) and was able to clear unwanted anionic cellular remnants such as microparticles from the circulation. The scavenger function of b2-GPI is dependent on the stretched conformation. After binding of LPS, b2-GPI is cleared by monocytes. The new indications regarding a possible physiological role of b2-GPI could also shed light on the events that cause the formation of auto-antibodies against the protein, and why auto-antibodies against this protein results in an increased risk for thrombo-embolic complications and fetal losses.